Brothers Josep Antoni and Víctor Grífols i Lucas had been investigating the process of lyophilization and its application to human plasma since 1935.They introduced the patented procedure to Spain in 1943, and Víctor was in charge of developing a lyophilizer to capitalize on the investment.
Overcoming scarcity with ingenuity
Due to the scarcity of materials during the post-war period in Spain, the first prototype designed by Víctor Grífols i Lucas was made using second-hand components, such as a vacuum machine from a light bulb factory. But to verify that it worked, it was necessary to use a very precise vacuum meter, a device that did not exist in Spain at the time. Víctor commissioned it from a local company, providing all the necessary calculations to manufacture the instrument.
The next step was to test the pumping mechanism. The first attempts were a failure, and did not reach the minimum vacuum necessary. They lost a lot of time trying to adjust the device, until in the end they discovered the cause: the oil the vacuum pump had to be submerged in to prevent air from entering through tiny holes contained volatile compounds. These chemicals were seeping into the device and preventing a vacuum from forming.
From hot oil to dry ice
The solution was to heat the oil over a primus stove to evaporate the offending compounds. Víctor Grífols spent the night with an extinguisher by his side trying not to fall asleep and risk setting the house on fire. At six o'clock in the morning he turned off the stove and let the oil rest. Once it was cold, he started up the pump–which now created a vacuum.
The final part of the puzzle was refrigeration. It had to be moisture-free, so dry ice was used, but later, a refrigeration compressor proved more practical. Ideally, a temperature of minus 70º Celsius was needed for the process. But the materials available at the time would only let them achieve minus 40º Celsius. They overcame the problem by slowing the entire process down – and finally it worked.
Patience and innovation are rewarded
After overcoming all the difficulties that arose when building the lyophilizer, Laboratorios Grífols became the first to lyophilize plasma in Spain. The lyophilized plasma could be stored at room temperature for five years, the shelf-life established by the American pharmacopoeia. That longevity is a vast improvement on just 21 days for fresh liquid plasma, and illustrates just how important the production of the Grifols lyophilizer was. The process proved extremely popular, and in fact years later, Nestlé would come to Grifols to learn the technique that was being used with plasma and apply it to the manufacture of instant coffee.
In order to sell it to hospitals throughout Spain, Grifols set up the beginnings of a sales network, whose first representative was Juan Taberner, in Alicante.
Overcoming shortages with ingenuity, innovation and total determination